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(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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Servicebio Inc antibodies against ikkα + β
(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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GeneTex ikk-α/β antibody
(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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Wanleibio antibody against nf-κb kinase α/β (ikkα/β)
(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, <t>p-IKK,</t> and IKK. (C) Protein expression of FAS, ACC, <t>and</t> <t>CPT1α</t> were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.
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Image Search Results


(A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, p-IKK, and IKK. (C) Protein expression of FAS, ACC, and CPT1α were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.

Journal: PeerJ

Article Title: Foxa2 attenuates steatosis and inhibits the NF-κB/IKK signaling pathway in nonalcoholic fatty liver disease

doi: 10.7717/peerj.16466

Figure Lengend Snippet: (A) Protein expression of Foxa2. (B) Protein expression of p-NF-κB, NF-κB, p-IKK, and IKK. (C) Protein expression of FAS, ACC, and CPT1α were detected by western blotting. HepG2 cells were treated with OA, oe-Foxa2, and lipopolysaccharide (LPS, 1 µg/mL). ** P < 0.01 compared with Control; ## P < 0.01 compared with OA. & P < 0.05 and && P < 0.01 compared with OA + oe-Foxa2 + LPS.

Article Snippet: The primary antibodies including carnitine palmitoyl transferase 1α (CPT1α; 1:1000; DF12004), Foxa2 (1:1000; DF13363), NF-κB (1:2000; AF5006), p-NF-κB (1:2000; AF2006), IKK (1:2000; AF6014), and p-IKK (1:2000; AF3013) were purchased from Affinity Biosciences (JIangsu, China); acetyl-CoA carboxylase (ACC; 1:1000; 3676) and GAPDH (1:1000; 2118) were purchased from Cell Signaling Technology (Waltham, MA, USA); fatty acid synthase (FAS; 1:1000; E-AB-40063) was purchased from Elabscience Biotechnology Co., Ltd (Wuhan, China).

Techniques: Expressing, Western Blot, Control